Enumeration and comparative distribution of coliphages in foods

Enumeration and comparative distribution of coliphages in foods by James E. Kennedy Download PDF EPUB FB2

ENUMERATION AND COMPARATIVE DISTRIBUTION OF COLIPHAGES IN FOODS By James E. Kennedy, Jr. December, Chairperson: James L. Oblinger Co-Chairperson: Cheng-i Wei Major Department: Food Science and Human Nutrition Studies were conducted to develop expedient, rapid and efficient methods for enumeration of coliphages in foods and to investigate the.

Complex basal eluents, EC medium and 1% casein, were generally more effective than a less complex eluent, phosphate buffer, for elution of coliphages from foods. For most foods, incorporation of sodium chloride or chaotropic agents, i.e., sodium trichloroacetate, urea, Tw Triton X, and sodium nitrate, into basal eluents did not enhance recovery of by: Abstract.

Five methods for the enumeration of micro-organisms in food (pour plate, surface spread plate, surface drop, agar droplet, and microdilution) were used in parallel to examine samples selected from a wide range of food products.

Statistical analyses of the results showed that the regression and correlation coefficients between Cited by: Coliphages recovered from 38 samples of ten different food products were characterized with regard to temperature of infec­ tivity.

High temperature (HT) phages were capable of reproduc­ tion at or above 30°C, mid temperature (MT) phages over a range of 20 to. Two groups of bacteriophages that infect E. coli, somatic and F-specific coliphages, have been used as both fecal and viral indicators in academia for many years [1,2,3,4].The high concentration of coliphages found in raw wastewater and many other matrices contaminated with fecal remains, the easy, fast, and cost-effective detection and enumeration methods, their persistence in the water Cited by: review primarily focuses on coliphages because there is more literature available on their occurrence, fate, and epidemiological relationships.

Additionally, two standardized enumeration methods published by EPA are available for both coliphages. Background. For over a century, FIB (i.e., total coliforms, fecal coliforms, E. coli. The standard method for the enumeration of somatic coliphages in water proposed for over a decade by the APHA (Anon,Anon, ) recommends sampling 5 ml water in single layer plaque assays, using frozen and thawed E.

coli C cultures as bacterial host. Although rapid thawing (at °C) of frozen cultures is recommended, Cited by: 4. Enumeration of micro-organisms in food: a comparative study of five methods BY J. KRAMER AND R. GILBERT Food Hygzene laboratory, Central Public Health Laboratory, Cotindale Avenue, London NW9 5H1P, Enyland (Recesved 23 December ) SUMMARY Five methods for the enumeration of micro-organisms in food (pour plate.

coliphages per mL bulk reagent water sample to achieve the target spike concentration of approximately 80 PFU / sample and swirl to mix. Aliquot each bulk sample into four, mL samples (see Section 11 for enumeration of coliphage stock and Section for.

performed during the bacterial enumeration lab. Dilutions of phage lysate are plated onto a soft agar lawn of E. coli cells. A viral particle will infect a single host where it lands. It will then reproduce, exit the host during the lytic cycle, and diffuse to infect nearby host cells.

The result is a clearing of E. coli cells known as a plaque File Size: 34KB. James E. Kennedy has written: 'Enumeration and comparative distribution of coliphages in foods' -- subject(s): Bacteriophages Asked in Authors, Poets, and Playwrights What has the author E J Rice.

Food and Drug Administration Enumeration of Somatic Coliphages. Geneva This work aimed to understand the distribution of five bacterial pathogens in O'ahu coastal streams and relate their.

Abstract. The count of coliphages in naturally polluted waters was found to be dependent on many experimental factors. If Escherichia coli C was used as a host strain, consistently higher counts were obtained than with other strains (B, Kderivatives). This could be explained partly by the absence of a restriction system in by: Havelaar et al.

() examined the distribution of fecal indicator bacteria and coliphages (both somatic and F-specific) in fecal samples and a variety of wastewaters.

Somatic coliphages were abundant in all fecal samples, whereas F-specific coliphages were seldom found in feces from humans or cattle, but abundant in pig and bird fecal matter. Bacteriophages are viruses that infect bacteria, those that specifically infect E. coli and other coliforms are termed coliphages.

While not a threat to humans, occurrence of somatic and F-RNA coliphages has been correlated with the presence of human enteric viruses in faecal-polluted water environments.

This study focused on investigating the incidence of somatic coliphages and F-RNA Author: Theodora Ijeoma Ekwomadu, Nomathamsanqa Patricia Sithebe.

Alcuni microrganismi possono essere impiegati come indicatori della qualità microbiologica degli alimenti, in relazione sia alla durata dei prodotti, sia all’assenza di agenti patogeni. Part of the Food book series (FOOD) Estratto () Methodology for enumeration of coliphages in foods. Appl Environ Microbiol, – Google.

Water quality through the presence of pathogenic enteric microorganisms may affect human health. Coliform bacteria, Escherichia coli and coliphages are normally used as.

The presence of unvaccinated free-roaming dogs (FRD) amidst human settlements is a major contributor to the high incidence of rabies in countries such as India, where the disease is endemic. Estimating FRD population size is crucial to the planning and evaluation of interventions, such as mass immunisation against rabies.

Enumeration techniques for FRD are resource intensive and can vary from Cited by: 4. Coliphages are generally found in the gut and are excreted in the feces of humans and other warm-blooded animals. Coliphages are present in large numbers in sewage (approximately plaque forming units [PFU] per milliliter [mL]) (Ewert and Paynter.

*Allows isolation and enumeration of discrete colonies of bacteria. *Provides presence or absence information within 24 hours.

*Effective and acceptable technique. Used to monitor drinking water in government laboratories. *Useful for bacterial monitoring in the pharmaceutical, cosmetics, electronics, and food and beverage industries. This part of ISO specifies a method for the detection and enumeration of somatic coliphages by incubating the sample with an appropriate host strain.

The method is applicable to all kinds of water, sediments and sludge extracts, where necessary after cturer: Multiple.

Distributed through American National Standards Institute.Distribution comparison between coliphages and phages of anaerobic bacteria (Bacteroides fragilis) in water sources and their reliability as fecal pollution indicators in drinking water. Water Sci.

&. The Escherichia coli host strain CB was tailored to detect both somatic and F-specific coliphages in a single test. The efficacy of this strain for bacteriophage detection, previously evaluated Author: Claudia Campos, Javier Méndez, Camilo Venegas, Luisa Fernanda Riaño, Paula Castaño, Natalia Leiton.